艾维库林治疗通过NLRP3通路调节微质极化及其外体,改善缺血性中风损伤
Yan Shi1, Yufeng Yang2, Juntong Liu3
1Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, 110032 Shenyang, Liaoning, China.
Journal of integrative neuroscience
|November 29, 2024
概括
在缺血性中风模型中,阿维林 (AL) 通过通过NLRP3通路和微质外体调节微质极化来减少脑损伤和炎症. 这种天然化合物为中风恢复提供了潜在的治疗策略.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 来自Banxia的avicularin (AL) 具有与大脑疾病相关的抗炎作用.
- 虽然AL会影响巨细胞的两极分化,但它在缺血性中风 (IS) 损伤中的作用仍然未被探索.
研究的目的:
- 在缺血性中风模型中研究阿维林 (AL) 的保护作用和潜在机制.
- 为了确定AL是否调节微质偏振和NLRP3炎症酶激活在IS的背景下.
主要方法:
- 在体内研究涉及给中脑动脉封闭/再输 (MCAO/R) 的小鼠注射AL,并评估神经学分数,心脏病发作量和亡.
- 在体外实验中治疗了缺氧-葡萄糖 (OGD) -BV2细胞和患有AL的原发皮质神经元.
- 技术包括TUNEL测定,流细胞测量,ELISA,西斑,以及通过TEM和NTA分析微质外体.
主要成果:
- 在MCAO/R小鼠中,AL治疗显著减少了神经缺陷,心脏病发作大小,脑水含量和神经元亡.
- AL促进了M2微质极化,同时抑制了缺血半中M1极化.
- AL抑制了NLRP3炎症酶激活,并增强了微质外体对神经元的抗亡作用.
结论:
- 在IS模型中,阿维cularin (AL) 减轻了炎症反应和神经元死亡.
- 通过NLRP3通路调节微质极化,AL发挥神经保护作用.
- AL还影响微质外体的功能,有助于其在缺血性中风中的治疗潜力.
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