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在全血中直接检测多克索鲁比辛,使用一种用水凝保护的电化学阿普坦基生物传感器

Songjia Luo¹, Qingliu Wu¹, Lu Wang²

¹School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.

|November 29, 2024

概括

一种新的用水凝保护的基于aptamer的电化学生物传感器 (HP-EAB) 能够有效地检测到全血中的多克索鲁比辛. 这种稳定的生物传感器克服了生物污染问题,使复杂的生物流体能够进行准确的实时分析.

科学领域:

生物传感器技术技术
电化学电化学电化学
生物标志物检测检测生物标志物检测

背景情况:

基于电化学体的生物传感器 (EAB) 提供实时检测,但由于生物污染,在复杂的生物流体中难以获得灵敏度和选择性.
生物分子在aptamer探针和电极上的无活化和吸附限制了EAB在全血样本等样本中的应用.

研究的目的:

开发一种简单的水凝保护的阿普坦基生物传感器 (HP-EAB),用于在全血中直接和准确检测多克索鲁比 (DOX).
提高EAB在复杂的生物矩阵中的稳定性和减少非特异性反应.

主要方法:

用于基于电化学aptamer的生物传感器的糖凝涂层电极的制造.
利用阿帕特马选择性来检测多克索鲁比.
在稀释全血和子全血样本中测试传感器的性能.

主要成果:

在7天的储存后,HP-EAB显示出高稳定性,并保持了优异的检测性能,与未受保护的EAB不同.
水凝涂层有效地减少了全血基质的非特异反应,延长了传感器的寿命.
传感器在子全血中实现了25.9nM的检测极限和0.1μM50μM的DOX检测范围.

结论:

开发的HP-EAB为复杂生物流体中分析物的敏感和选择性检测提供了一个强大的平台.
水凝保护是一种可行的策略,可以提高EAB在现实生物样本中的稳定性和适用性.
这种方法可以在全血中直接快速准确地确定重要的生物标志物.

关键词:

抗生物污染的抗生物污染剂多克索鲁比检测检测方法基于电化学阿普他默的生物传感器.水凝是一种水凝.这是全血,全血.

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