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作为不孕不育的潜在预测因素的DNA断点和自由DNA片段

Yue Zhou¹, Juan Wang^1,2, Fan Zhang³

¹Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China.

Scientific reports

|November 29, 2024

概括

变水显著增加了精子DNA损伤,通过DNA碎片化指数 (DFI),精子DNA破裂的平均数量 (MDB) 和精液等离子体中的自由DNA碎片 (fDFA) 来衡量. 结合MDB和fDFA在男性不孕症病例中提供了更有效的生育预测.

科学领域:

生殖医学生殖医学
安德罗学与人类学
遗传学是一个遗传学.

背景情况:

男性不孕症影响了试图怀孕的夫妇的很大一部分.
静脉是男性不孕症的常见原因,通常与精子质量受损有关.
精子DNA完整性对于成功受精和胚胎发育至关重要.

研究的目的:

为了研究白血球菌对精子DNA完整性的影响.
为了比较DNA碎片化指数 (DFI) 的预测值,精子DNA断裂的平均数量 (MDB) 和精液等离子体中的自由DNA碎片 (fDFA) 对于与瘤相关的男性不孕症.
探索MDB和fDFA在评估男性生育能力方面的联合疗效.

主要方法:

分析了来自42名静脉患者和22名健康对照者的精子样本.
评估了精子的基本参数 (度,活力).
精子DNA碎片化指数 (DFI) 使用SCSA方法进行测量.
精子血中精子DNA断裂 (MDB) 和自由DNA片段 (fDFA) 的平均数量使用TDT-Strand Displacement Probe技术进行了分析.

主要成果:

与健康个体相比,静脉患者的DFI,MDB和fDFA水平显著更高.
与单独的DFI或个人评估相比,MDB和fDFA的组合显示出更好的生育预测能力.
升高的MDB和fDFA水平与瘤诱导的男性不孕症密切相关.

结论:

MDB和fDFA显示出作为有价值的生物标志物来评估男性生育能力在瘤的背景下有希望.
对MDB和fDFA的综合评估可能会为瘤相关的不孕症提供更强大的诊断工具.
需要进一步的研究来验证这些发现,并为在男性不孕症管理中使用MDB和fDFA制定临床指南.

关键词:

精液等离子体中的自由DNA 基因突破点的平均数量这就是ROSOS ROS.精子分析精子分析这种病的发病率是Varicocele.

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