用血激活水 (PAW) 杀死的Bacillus cereus子的特征
Xiao Hu1, Pengfei Ge1, Xiaomeng Wang2
1School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Food research international (Ottawa, Ont.)
|November 30, 2024
概括
血激活水 (PAW) 通过破坏它们的外层和内部结构,有效地使Bacillus cereus子失活. 这一过程损害了子耐药性,提供了一种新的微生物控制方法.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 生物技术是生物技术.
背景情况:
- 细菌花 (B. cereus) 子是微生物污染的一个重大问题.
- 血激活水 (PAW) 是微生物无活化的有前途的技术.
研究的目的:
- 调查PAW对B. cereus子产生的结构和功能损伤.
- 阐明PAW介导的子无活化背后的机制.
主要方法:
- 对B. cereus子暴露于PAW.
- 显微镜分析 (SEM,TEM) 来观察形态变化.
- 生物化学测试用于评估子外完整性,膜透性,蛋白质变性,DNA完整性和酶活性.
- 脂肪酸组成和脂质过氧化的分析.
- 使用Young的模量进行机械性能评估.
- 子组成部分的光谱分析 (拉曼).
主要成果:
- PAW诱导了二二二酸 (DPA) 的释放,并损害了子外,内膜和皮质.
- 观察到显著的形态变化,包括纹,痕和形状变化.
- PAW处理降低了子的弹性,并降低了对各种应力 (NaClO,NaCl,热,UV,H2O2,溶酶) 的抵抗力.
- 生物化学分析显示蛋白质变性,DNA损伤,SASP降解和抑制酶活性.
- 脂肪酸组成,脂质过氧化和拉曼光谱的变化证实了PAW对子活力的有害影响.
结论:
- PAW有效地向B. cereus子的多个组成部分,导致不活化.
- 观察到的结构和生化损伤解释了PAW对子的抵抗性降低和不活化.
- PAW提出了一种可行的非热方法,用于控制B. cereus子污染.
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