猪肉肌纤维蛋白的结构,聚合行为和凝性质的变化是由theaflavins诱导的
Chunlin Nie1, Junyi Xiang2, Jiabao Zheng1
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Food research international (Ottawa, Ont.)
|November 30, 2024
概括
茶叶黄素 (TFs) 对猪肉肌纤维蛋白 (MP) 凝能力产生负面影响. TFs通过干扰二硫化物键形成来降低MP凝强度和性能.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 肉类科学 肉科学
背景情况:
- 肌纤维蛋白 (MP) 对肉质和凝形成至关重要.
- 茶叶中发现的多烯,即茶叶烯 (TFs),以其抗氧化特性而闻名.
- 了解TF与MP的相互作用对于食品加工和质量很重要.
研究的目的:
- 调查不同度的theaflavin (TF) 对猪肉肌纤维蛋白 (MP) 结构,聚合和凝的影响.
- 阐明底层的TF-MP相互作用的分子机制.
- 评估TFs对MP的功能性质的影响.
主要方法:
- 使用自由硫含量,表面水性,光谱学,粒子大小分析和SDS-PAGE分析的MP结构和聚合.
- 凝性能通过凝强度,损耗,微观结构分析和凝超水剂的SDS-PAGE进行评估.
- 测试的TF度在0至900mg/L之间.
主要成果:
- 增加TF度导致自由硫醇含量显著下降,表明TF和MP硫醇组之间存在共价相互作用.
- 内在光谱检测显示了静态火,这表明TF-MP复合体的形成.
- TFs减少了MP粒子大小,防止了SDS-PAGE中的聚合,降低了凝强度,增加了损失.
- 凝超浮物的SDS-PAGE显示,TFs干扰了对凝至关重要的二硫化键形成.
结论:
- 茶叶黄素与猪肉肌纤维蛋白相互作用,导致醇损失并改变蛋白质结构.
- TFs通过破坏凝网络形成所需的二硫化物交叉链接来损害MP的凝能力.
- 这些发现表明,当TFs作为添加剂使用时,可能会对肉类产品的质地和质量产生负面影响.
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