工程微环境和胰腺小岛芯片用于选糖替代品和抗糖尿病化合物
Jingyan Shi1, Jianing Li1, Wentao Su2
1Materials Genome Institute, Shanghai University, Shanghai 200444, China.
Food research international (Ottawa, Ont.)
|November 30, 2024
概括
一个新的胰腺小岛芯片系统增强了细胞活力和功能,用于研究食品添加剂. 这个平台准确地评估糖替代品和潜在的抗糖尿病化合物,揭示了对胰岛素分泌动态的洞察力.
科学领域:
- 生物技术和生物医学工程 生物技术和生物医学工程
- 内分泌学和新陈代谢学
- 食品科学和毒理学 食品科学和毒理学
背景情况:
- 对食品添加剂安全性的日益关注,需要先进的模型来预测生物效应.
- 现有的静态培养方法限制了对食物成分的动态细胞反应的准确评估.
- 需要复杂的平台来评估糖替代品和其他添加剂对胰腺小岛功能的影响.
研究的目的:
- 开发和验证一种创新的胰腺芯片上的岛屿系统,以更好地预测食品添加剂的影响.
- 用开发的芯片研究特定食品添加剂 (包括糖替代品) 对胰岛素分泌的影响.
- 建立一个动态的3D细胞培养环境,模仿胰腺伪岛的生理条件.
主要方法:
- 设计了一个芯片上的小岛系统,包括度梯度发生器和可 perfusable 3D 细胞培养阵列.
- 在动态微环境中培养胰腺伪岛,将结果与静态培养条件进行比较.
- 量化小岛细胞活力,集群大小,聚合和关键小岛发育因子 (INS-1,INS-2,PDX-1) 的表达水平.
主要成果:
- 动态培养系统保持了超过95%的小岛细胞活力,促进了更大的细胞群和聚合.
- 在动态环境中观察到INS-1 (4.5倍),INS-2 (1.9倍) 和PDX-1 (5.8倍) 表达的显著增加.
- 糖替代品 (氨酸,糖) 没有影响胰岛素分泌,而GLP-1,exendin-4和黄素显著增加了胰岛素分泌 (p < 0.01).
结论:
- 开发的胰腺芯片上的岛屿系统为评估食品添加剂提供了强大而生理相关的平台.
- 该模型准确地区分了各种化合物对胰岛素分泌的影响,区分了无影响的糖替代品和生物活性剂.
- 工程微环境和芯片上的小岛技术代表了对抗糖尿病化合物和食品成分的临床前查的重大进步.
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