在Lactiplantibacillus plantarum亚种期间绘制天然叶酸的生物合成途径. 紫菜中的植物发酵通过转录基因分析
Mei Tan1,2, Xu Yan1, Hosahalli Ramaswamy3
1Department of Food Science and Technology, Jinan University, Guangzhou, China.
Journal of the science of food and agriculture
|November 30, 2024
概括
乳植物菌种植物菌种子. 植物在紫色卷心菜汁中产生叶酸,特别是5-甲基四基叶酸 (5-MTHF). 基因表达分析揭示了参与叶酸生物合成的关键基因,证实了该途径的存在.
科学领域:
- 微生物学 微生物学
- 营养科学 营养科学
- 生物化学 生物化学
背景情况:
- 叶酸对于许多代谢过程至关重要,作为单碳循环供体.
- 乳植物菌种植物菌种子. 植物因其叶酸合成能力而受到研究.
- 了解发酵食品中的叶酸生物合成对于营养增强至关重要.
研究的目的:
- 探索L. plantarum亚种的叶酸合成能力. 不同的媒介中的足部.
- 通过转录学学研究基因机制,研究叶酸生物合成的基因机制.
- 确定参与叶酸生产的关键基因和转录因子.
主要方法:
- 在L. plantarum亚种的发酵过程中. 在MRS和紫色卷心菜汁 (PCJ).
- 总叶酸和5-甲基四基叶酸 (5-MTHF) 含量的定量.
- 转录组分析用于研究发酵期间的基因表达模式.
主要成果:
- 在PCJ中观察到总叶酸增加了44%,其中5-MTHF占了很大一部分.
- 在MRS介质中没有检测到叶酸的产生.
- 关键叶酸生物合成基因 (aroC, pabA, folE, folA,MTHFR) 的升调,MTHFR显示8.4倍的增加.
结论:
- 在L. plantarum亚种中. 植物拥有完整的5-MTHF生物合成途径.
- 发酵条件显著影响叶酸的生产.
- 这些发现支持优化发酵以提高食品中叶酸含量.
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