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利用下一代测序在基于废水的流行病学和全球疾病监测中的力量

Kata Farkas¹, Rachel C Williams^2,3, Luke S Hillary⁴

¹School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK. fkata211@gmail.com.

Food and environmental virology

|November 30, 2024

概括

使用下一代测序 (NGS) 的基于废水的流行病学 (WBE) 可以增强病原体监测. 将NGS整合到常规的WBE程序中,可以改善对新出现的传染病威胁的早期检测,以更好地应对流行病的准备.

科学领域:

环境微生物学环境微生物学
公共卫生监督是对公共卫生的监督.
基因组流行病学基因组流行病学

背景情况:

基于废水的流行病学 (WBE) 是监测社区感染的强大工具,包括无症状病例.
下一代测序 (NGS) 为废水中病原体检测和遗传分析提供了先进的功能.
目前的WBE程序往往没有充分利用NGS的全部潜力来识别新的和已知的病毒威胁.

研究的目的:

批判性地评估常见NGS工具对于常规WBE的适用性.
评估NGS在检测具有流行病或流行病潜力的新兴传染病威胁方面的能力.
为加强全球监督提供将NGS整合到WBE的建议.

主要方法:

审查当前的NGS技术及其在WBE中的应用.
评估NGS用于变种和重组基因型识别 (例如,SARS-CoV-2,脊髓灰质炎病毒).
在废水样本中检测已知和新型病毒的NGS的评估.

主要成果:

在WBE中,NGS使得除了定量病原体评估之外,可以进行详细的分析.
许多先进的NGS工具在常规WBE程序中仍未得到充分利用.
国家污水系统显示了识别废水中新出现的病毒威胁的巨大潜力.

结论:

将NGS集成到WBE可以显著提高新出现的传染病威胁的检测.
提供了基于证据的建议,用于在WBE计划中采用NGS.
在WBE中使用NGS支持在One Health框架内做出明智决策,以改善疫情防控.

关键词:

一个健康一个健康流行病的准备情况.病毒学是病毒学.废水监测废水监测废水监测废水的排序排序是如何进行的动物传染病是动物传播的疾病.

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