相关实验视频

Updated: Jun 6, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

在急性髓性白血病的进展中,HOX嵌入的非编码RNA的分子杆作用

Christine Wilson¹, Priyanka Swaroop², Sachin Kumar¹

¹Department of Medical Oncology, Dr. B.R.A. IRCH, All India Institute of Medical Sciences, Room No. 401, 4th Floor, New Delhi, India.

|November 30, 2024

概括

这项研究回顾了Homeobox (HOX) 基因集群中的非编码RNA (ncRNA) 如何驱动急性髓性白血病 (AML). 这些HOX ncRNAs,特别是来自HOXA和HOXB集群的HOX ncRNAs,是白血病发生的关键,并提供潜在的治疗点.

科学领域:

* 分子生物学 * 分子生物学
* 癌症研究研究
* 遗传学在遗传学方面

背景情况:

*急性骨髓性白血病 (AML) 是一种异质癌症,骨髓细胞分化受损.
* AML的分子驱动因素尚未完全理解,这阻碍了向治疗.
*Homeobox (HOX) 基因对细胞分化至关重要,它们的失调与AML有关.

研究的目的:

*全面审查涉及AML的HOX嵌入型非编码RNA (ncRNA) 的文献.
*阐明HOX ncRNAs对白血病发生的作用机制.
* 探索HOX ncRNAs在AML中的治疗潜力和生物标记器实用性.

主要方法:

*对HOX嵌入的ncRNAs (lncRNAs,miRNAs,cirRNAs,piRNAs) 在AML中的综合文献综述.
*对HOXncRNAs对HOX基因表达和白血病发生的调节作用的分析.
* 检查异常的HOX ncRNA表达在特定的AML亚型,如NPM1突变的AML.

主要成果:

*HOX基因集群编码功能性ncRNA,包括在AML中起作用的lncRNA和miRNA.
*主要来自HOXA和HOXB的HOX ncRNAs调节HOX转录因子表达,促进白血病发生.
*HOX ncRNAs可以调节相同或不同的染色体上的基因,从而影响复杂的调节网络.
*从HOX基因集群编码的循环RNA和piwiRNA尚未在AML中确定.

结论:

*来自HOX集群的ncRNAs是AML的关键调节者,提供了新的治疗点.
*HOX ncRNAs的异常表达,特别是在NPM1突变的AML中,突出了它们作为预后生物标志物的潜力.
* 针对HOX ncRNA网络是一个有前途的策略,可以解决AML的分子异质性.

关键词:

急性骨髓性白血病 (AML) 是一种急性骨髓性白血病.生物标志物生物标志物在HOX非编码RNA中.在 lncRNAs 中.微RNAs 是一个微型RNA.

更多相关视频

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

相关概念视频

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...