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乙支柱酸盐对骨代谢的影响基于网络毒理学和分子对接技术

Xuan Lin¹, Kun Lin², Yue Lai³

¹Central Laboratory, Affiliated Hospital of Putian University, Putian, Fujian Province 351100, China; Department of Environmental and Biological Engineering, Putian University, Putian, Fujian Province 351100, China.

Ecotoxicology and environmental safety

|November 30, 2024

概括

乙支柱酸盐 (ATBC) 通过降低关键表观遗传调节者的调节,损害骨质细胞功能和骨代谢. 这项研究揭示了将ATBC暴露与潜在的骨科疾病联系在一起的分子机制.

科学领域:

环境健康环境健康
分子生物学分子生物学
骨的新陈代谢骨的新陈代谢

背景情况:

环境污染物,如乙三酸 (ATBC) 可以影响人类健康.
了解ATBC对骨代谢影响的分子基础对于公共卫生至关重要.

研究的目的:

阐明ATBC对骨代谢影响的分子机制.
为了确定关键的分子点和受ATBC影响的信号通路.

主要方法:

生物信息分析 (ChEMBL,STITCH,GeneCards,OMIM,STRING,Cytoscape,Metascape) 用于确定ATBC的点和途径.
分子对接模拟 (AutoDock) 来确认ATBC-目标相互作用.
在体外实验中使用暴露于ATBC的骨质母细胞 (MC3T3-E1).

主要成果:

确定了十个核心分子标和几个关键信号通路 (例如甲状腺激素,FOXO,胰岛素).
低剂量的ATBC (100μM) 损害了骨质母细胞的增殖和迁移.
在骨质母细胞中,ATBC暴露降低了关键表观遗传调节剂 (KDM1A,EP300,HDAC2) 的下调.

结论:

通过影响表观遗传调节剂,ATBC会破坏细胞水平的骨代谢.
这项研究为了解ATBC诱导的骨疾病提供了一个分子框架.
结果可能会为预防与ATBC暴露相关的骨科问题制定策略.

关键词:

在ATBC中,ATBC就是ATBC.骨的新陈代谢骨的新陈代谢分子对接是分子对接.网络毒理学网络毒理学

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