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与抵抗洋Fusarium基底腐烂有关的与防御相关的酶

SaeidReza Poursakhi¹, Hossein Ali Asadi-Gharneh¹, Mehdi Nasr-Esfahani²

¹Department of Horticulture, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

Plant physiology and biochemistry : PPB

|November 30, 2024

概括

耐药洋品种显著增强了抗氧化酶 (catalase,过氧化酶,超氧化脱酶) 和防御基因表达,当与Fusarium oxysporum f. sp.挑战时. 塞佩亚 (FOC) 公司. 这种分子反应是它们增强的抗病能力的基础.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
生物化学生物化学

背景情况:

富沙氧化 (Fusarium oxysporum) f. sp. 这种植物有很多种. cepae (FOC) 导致洋产量大幅下降.
了解洋抵抗FOC的分子机制对于开发耐药品种至关重要.
抗氧化酶和特定的转录因子在植物防御反应中起作用.

研究的目的:

量化抗氧化酶活性 (CAT,POX,SOD) 在耐药和易受性洋品种在FOC接种后的变化.
评估对FOC的反应中关键的防御相关转录因子 (R1,PR5,RGA29) 的表达水平.
为了将分子变化与疾病严重程度和生物质增长参数相关联.

主要方法:

在幼苗阶段用FOC接种了具有差异性FOC耐药性的洋品种.
测量了催化酶 (CAT),过氧化酶 (POX) 和超氧化物失调酶 (SOD) 的活性.
使用定量实时PCR (qRT-PCR) 来确定R1,PR5和RGA29基因的表达.

主要成果:

耐药品种 ("Saba - HS", "Saba") 与易感品种 ("Golden Eye", "Savannah Sweet" - 88.9-89.6%) 相比,疾病严重程度明显较低 (4.7-6.7%).
耐药品种显示了CAT (4.22-4.81倍),POX (2.35-3.53倍) 和SOD (17.46-22.95倍) 活动的显著上调.
在耐药基因型中,防御基因RGA29,R1和PR5的表达分别上调3.83,4.78和5.01倍.

结论:

抗氧化酶的增强活动和与防御相关的转录因子的上调是洋对FOC抗性的关键组成部分.
该研究确定了与洋中FOC耐药性相关的特定分子标记物 (CAT,POX,SOD,R1,PR5,RGA29).
这些发现为培育对Fusarium基底腐烂的抗性提高的洋提供了分子基础.

关键词:

生物质是生物质中的一部分.与防御相关的标记基因与防御相关的标记基因.氧化降解酶是一种氧化降解酶.这就是ROSOS ROS.qRT-PCR 是一个很好的方法.

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