在宿主内部传染的标志 Mycobacterium结核病复杂变异:一个回顾性基因组流行病学研究研究
Katharine S Walter1, Ted Cohen2, Barun Mathema3
1Division of Epidemiology, University of Utah, Salt Lake City, UT, USA.
The Lancet. Microbe
|November 30, 2024
概括
宿主内Mycobacterium结核病综合体 (MTBC) 变异,或感染个体内的遗传多样性,可以提高重建传播链的准确性. 分析这种宿主内部变异为了解结核病 (TB) 传播提供了有价值的工具.
科学领域:
- 基因组流行病学基因组流行病学
- 微生物进化过程中的微生物.
- 传染病的传播是传染病的传播.
背景情况:
- 结核菌菌复合体 (MTBC) 进化缓慢,导致类似的基因组阻碍传播链重建.
- 已经报告了宿主内部的MTBC变异,但其在改善传输推断方面的实用性仍然不清楚.
研究的目的:
- 为了量化宿主内部的MTBC变化.
- 评估主机内部变异数据是否可以提高传输推断的准确性.
主要方法:
- 用公共全基因组测序和来自家庭传播研究的流行病学数据进行回顾性基因组流行病学研究.
- 在样本内和样本之间的PE/PPE基因之外的少数变异的量化.
- 用ROC曲线来预测家庭成员数量的一般线性模型与没有共享的少数变体进行比较.
主要成果:
- 分析了三项MTBC家庭传播研究,揭示了少数群体变异的中等水平.
- 来自家庭对的分离物与随机对相比,共享的少数变体明显更多.
- 包含宿主内部共享变化的模型在所有研究中都在预测家庭成员的准确性上得到了改进.
结论:
- 宿主内部的MTBC变异通过培养持续存在,并可以增强传输推断的分辨率.
- 恢复变异的差异凸显了需要优化方法来将宿主内部的变异纳入遗传学和传播分析.
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