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在Rpptortus pedestris (Hemiptera: Alydidae) 中对通关通路基因的全转录组识别和表征

Yipeng Ren¹, Wenhao Dong², Juhong Chen¹

¹Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.

Developmental and comparative immunology

|November 30, 2024

概括

这项研究研究了大豆害虫Riptortus pedestris的Toll路径,揭示了非编码RNA如何调节免疫基因,以应对肠道细菌和环境变化对害虫控制策略的反应.

科学领域:

昆虫免疫学昆虫免疫学
分子生物学分子生物学
害虫管理害虫管理害虫管理

背景情况:

收费通路对于昆虫对病原体的免疫非常重要.
非编码RNAs (ncRNAs) 调节昆虫的免疫恒温.
托尔通路基因和ncRNAs在Rpptortus pedestris肠道适应中的作用尚不清楚.

研究的目的:

为了表征Riptortus pedestris中的收费通路基因.
研究ncRNAs对环境和肠道细菌变化的反应中的转录后调节.
探索针对收费通道的潜在害虫控制策略.

主要方法:

六个Toll基因的识别和分类.
分析跨发育阶段和养治疗的基因表达模式.
预测miRNA-mRNA相互作用和构建一个ceRNA网络.
皮尔森对基因/ncRNA表达和肠道细菌之间的相关性分析.

主要成果:

鉴定和分类了六个Toll基因,其表达变化与肠道环境有关.
构建了一个由60个miRNA-mRNA相互作用和两个lncRNA-miRNA-Toll对组成的网络.
基因和ncRNA表达显示与肠道细菌组成的关联.
收费通路基因在转录后层面参与环境适应.

结论:

在Riptortus pedestris中,Toll通路由ncRNAs调节,并响应肠道细菌的变化.
这为昆虫的适应和害虫控制的潜在目标提供了洞察力.

关键词:

肠道细菌是什么肠道细菌是什么没有编码的RNAs.害虫控制害虫控制害虫控制害虫控制害虫控制害虫控制里普托尔图斯 (Riptortus pedestris) 是一个步行者.收费通道上的收费路径.

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