骨髓衍生的树突细胞在减轻Bothrops jararacussu毒素肌肉损伤的炎症方面发挥着作用
N M Nery1, A A Ferreira E Ferreira1, H M Santana1
1Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
Journal of biotechnology
|November 30, 2024
概括
状细胞 (DCs) 减轻Bothrops jararacussu毒液 (BjV) 诱导的肌肉损伤. 用成熟的骨髓树突细胞 (BMDCs) 治疗中毒减少了炎症,促进了肌肉再生.
科学领域:
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
- 毒理学 毒理学 毒理学
背景情况:
- 树突细胞 (DCs) 是免疫系统的关键调节者,启动T细胞反应.
- DCs与其他免疫细胞一起在早期肌肉损伤中发挥作用.
- 博思罗普斯 (Bothrops jararacussu) 毒液 (BjV) 会导致严重的组织损伤和炎症.
研究的目的:
- 调查DCs在缓解肌肉损伤和促进BjV中毒后再生方面的潜力.
- 在BjV诱导的肌肉损伤模型中评估成熟的骨髓树突细胞 (BMDC) 的治疗效果.
主要方法:
- 给小鼠注入BjV的毒素,然后用LPS激活的BMDC注射到胃肌中进行治疗.
- 在7天的时间内评估了环境效应和再生,监测了组织损伤,白细胞涌入和炎症标志物.
- 测量了肌酸酶 (CK) 和细胞因子 (IL-6,TNF-α,IL-1β) 的血清和组织水平.
主要成果:
- BjV诱导了显著的组织亡,白细胞流入,出血,以及升高的CK和细胞因子水平.
- BMDC治疗减轻了局部损伤,减少了缩,出血和白细胞透.
- BMDC治疗降低了CK水平和促炎性细胞因子的释放,促进了中度肌肉再生.
结论:
- BjV会导致严重的局部炎症和肌肉损伤.
- 用BMDCs治疗对于最大限度地减少BjV诱导的肌肉损伤和炎症至关重要.
- BMDC的使用可促进肌肉组织在毒化后的早期修复和再生.
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