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基于SYNCAS的CRISPR-Cas9基因编辑在掠食性虫,白和臭虫中

A Mocchetti¹, S De Rouck¹, S Naessens¹

¹Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium.

Insect biochemistry and molecular biology

|November 30, 2024

概括

这项研究成功地适应了SYNCAS CRISPR-Cas9基因编辑方法用于掠食性虫和昆虫害虫,在Bemisia tabaci中实现了高编辑效率. 这些发现提升了对有益的节肢动物和农业害虫的基因操纵工具.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
昆虫学昆虫学是一门学科.

背景情况:

由于生物限制,CRISPR-Cas9基因编辑在许多关节动物中具有挑战性.
使用母体注射的SYNCAS方法在蜘蛛和虫中取得了成功.
对于有益的节肢动物和农业害虫,需要有效的基因编辑工具.

研究的目的:

评估SYNCAS方法在掠食性虫 (Amblyseius swirskii,Phytoseiulus persimilis) 和昆虫害虫 (Bemisia tabaci,Nezara viridula) 中基因编辑的适用性.
评估基因编辑效率,并观察目标节肢动物物种的结果表型.

主要方法:

孕产妇注射配制的CRISPR-Cas9成分 (沙素和BAPC) 进入目标节肢动物物种.
针对特定的基因:掠食性虫中的Antp和SLC25A38,以及B. tabaci和N. viridula.中的白色基因正基因.
基因型和表型分析以确认基因编辑和观察效应.

主要成果:

在所有测试物种中都取得了成功的基因编辑,在B. tabaci.中效率最高 (高达39%).
掠食性虫中SLC25A38的淘汰并没有产生可观测的表型,类似于蜘蛛虫.
在掠食性虫中破坏Antp似乎是致命的,阻止了零突变的恢复.
B. tabaci白色基因淘汰导致色眼睛,与D. melanogaster的表型不同.
N. viridula表现出一种低形态的等位基因,具有斑点的白色和野生类型的眼睛.

结论:

在A.swirski,P.persimilis,B.tabaci和N.viridula中,SYNCAS方法可以适应基因编辑.
这项研究为研究生物控制中的掠食性虫和半虫害提供了有价值的遗传工具.
需要进一步的研究来了解SLC25A38突变体缺乏表型以及Antp破坏的致命性.

关键词:

这是一只野兽,Amblyseius swirskii.贝米西亚烟草 (Bemisia tabaci) 是一种烟草.基因编辑基因编辑这是一次Knock-out.微注射微注射的方法尼扎拉病毒球 (Nezara viridula) 是一种病毒球.这种植物是Phytoseiulus persimilis.

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