使用无标签的NIR光吸收传感器检测C-反应性蛋白质,使用基于AIEE antracen衍生物的大量Stokes转移的NIR光吸收传感器
Zhuowei Fu1, Yiwen Yang2, Zhifeng Li2
1College of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China; Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, Zhejiang, China.
Analytica chimica acta
|November 30, 2024
概括
一种新型的食欲传感器使用近红外光检测C反应蛋白 (CRP). 这种方法为诊断与炎症相关的疾病提供了高灵敏度和特异性.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- C-反应蛋白 (CRP) 是炎症,心血管疾病和癌症的关键生物标志物.
- 准确的CRP检测对于疾病诊断和治疗至关重要.
- 现有的检测方法需要提高灵敏度和特异性.
研究的目的:
- 开发一种新的无标签的口味传感器,用于敏感和特定的CRP检测.
- 为了利用近红外 (NIR) 光与大斯托克斯转移增强检测.
- 整合聚合诱导的排放增强 (AIEE) 效应以改善信号.
主要方法:
- 开发了一种NIR光感应传感器,使用的是AIEE炭衍生物 (B化合物) 和二硫化 (MoS2) 平台.
- 化合物B与CRP吸附体 (CRP-Apt) 形成复合体,该复合体被吸附到MoS2上,从而灭光.
- 结合CRP与CRP-Apt将其从MoS2中释放出来,恢复B化合物的光.
主要成果:
- 感应器显示光恢复和CRP度 (0.3-70 ng/mL) 之间的线性反应.
- 对于CRP,已达到0.1 ng/mL的低检测极限 (LOD).
- 在人血清样本中成功应用了适应传感器来确定CRP.
结论:
- 开发的食感传感器为CRP检测提供了高灵敏度和特异性.
- 结合了NIR光,aptamer特异性和AIEE属性的优势.
- 为炎症相关疾病的临床诊断提供了一个有前途的工具.
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:19Protein Dynamics in Living Cells
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...