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通过使用响应表面方法来优化土著Trichoderma lixii和Talaromyces pinophilus对烯的生物降解

Samson O Egbewale¹, Ajit Kumar¹, Mduduzi P Mokoena²

¹Discipline of Microbiology, University of KwaZulu-Natal (Westville Campus), Durban 4000, South Africa.

Ecotoxicology and environmental safety

|December 1, 2024

概括

两种真菌,Trichoderma lixii和Talaromyces pinophilus,可以有效地生物降解. 使用响应表面方法 (RSM) 的优化实现了高降解效率和降低介质毒性,提供了可持续的PAH补救.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
菌生物技术是一种真菌生物技术.

背景情况:

多环芳 (PAH) 像炭一样,对环境构成风险.
本土真菌菌株为受污染地点的生物修复提供了潜力.
优化生物降解过程对于有效的环境清洁至关重要.

研究的目的:

为了优化由Trichoderma lixii FLU1 (TlFLU1) 和Talaromyces pinophilus FLU12 (TpFLU12) 进行烯生物降解的物理化学参数.
为了评估由选定的真菌菌株对二烯降解的效率和机制.
评估降解介质的毒性降低.

主要方法:

对土著真菌菌株进行查,以检测它们是否会发生烯生物降解.
优化参数 (pH,温度,生物质等) 的优化使用响应表面方法 (RSM) 与盒式设计 (BBD) 和中央复合设计 (CCD).
用Vibrio parahaemolyticus.鉴定降解代谢物和评估急性毒性.

主要成果:

TlFLU1和TpFLU12都显示出大量的烯生物降解潜力.
在特定条件下,RSM优化预测了高降解效率 (高达100%).
鉴定了代谢物9,10-氨基和酸;媒介毒性降低,尽管菌株被归类为有害.

结论:

使用RSM的优化条件显著增强了菌生物降解.
鉴定到的真菌菌株和优化过程显示了可持续的PAH修复的希望.
需要进一步的研究,以充分评估这些菌株的环境安全性和有效性.

关键词:

人类素 (Anthracene) 是一种生物降解生物降解红素溶解酶红素溶解酶红素分解酶在 RSM RSM 中.塔拉罗米克斯皮诺菲勒斯 (Talaromyces pinophilus) 是一种植物.在Trichoderma lixiixii中发现的

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