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波形变换辅助激光诱导的光探测器具有高灵敏度

Chao Zheng¹, Chuanliang Wang², Tao Sha³

¹School of Science, Dalian Maritime University, No. 1 Linghai Road, Ganjingzi District, Dalian, 116024, PR China; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-Sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China.

|December 1, 2024

概括

一种新的激光诱导光 (LIF) 探测器使用先进的光学和波波变换数据处理实现了超高灵敏度. 这种方法显著提高了超痕迹分析的信号噪声比,设定了新的检测极限.

科学领域:

分析化学分析化学
频谱学是一种光谱学.
生物物理学的生物物理.

背景情况:

传统的激光诱导光 (LIF) 探测器面临着灵敏度和降噪方面的挑战.
改善信号噪声比 (SNR) 和尽量减少污染对于超微量分析至关重要.
需要先进的光学设计和信号处理技术来提高LIF性能.

研究的目的:

开发和评估一个高度灵敏的LIF探测器,辅助波束变换.
提高光收集效率,减少LIF测量中的噪音.
建立新的,较低的检测极限 (LOD) 超痕分析剂.

主要方法:

使用一种非常规的显微镜镜像 (NA=0.5,WD=4.0毫米) 进行增强的光收集.
采用非球形镜头来最大限度地减少光成像大小,提高SNR.
在流量注入分析 (FIA) 中实施了"反向吸入注入"方法,以减少污染.
应用波波变换用于LIF信号处理以有效减少噪声.
结合LIF与毛细血管电泳 (CE) 进行最终灵敏度.

主要成果:

使用专用镜头实现了SNR大约5倍的增加.
进一步提高了SNR约1.3倍与非球状镜头.
通过波波变换而没有改变峰值特征,LIF信号噪声从0.009 mV降低到0.002 mV.
在FIA模式下为光素建立了9.7 × 10-14 M (3.9分子在68 pL) 的LOD.
当LIF与CE相结合时,光素的LOD达到4.9 × 10-15M.

结论:

开发的LIF探测器集成了先进的光学和波形变换,为超痕迹分析提供了前所未有的灵敏度.
"反向吸入注入"FIA方法在尽量减少污染错误方面被证明是有效的.
实现的LODs代表了FIA和CE模式中LIF检测的最低报告水平.

关键词:

良好的可复制性良好的可复制性高灵敏度的高灵敏度的高灵敏度逆向吸入注射的注射方式激光诱导的光是一种激光诱导的光.波段变换的波段变换是什么

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