来自Akkermansia属的扩展光谱β-乳酸酶的分子特征
Jiafu Lin1, Tiantian Wang1, Yaliang Zhou2
1Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of pharmacy, Chengdu University, 610106 Chengdu, China.
International journal of food microbiology
|December 1, 2024
概括
作为一种潜在的益生菌,Akkermansia muciniphila 具有β-lactamase 基因,使其对β-lactam 抗生素产生抗性. 这些基因似乎是不可转移的,支持Akkermansia.
科学领域:
- 微生物学 微生物学
- 益生菌研究 益生菌研究
- 抗生素耐药性 抗生素耐药性
背景情况:
- Akkermansia muciniphila 是一个关键的肠道微生物,也是一个有前途的下一代益生菌.
- 评估抗生素耐药性对于益生菌的安全性至关重要,但在Akkermansia物种中研究不足.
研究的目的:
- 为了研究阿克克曼西亚物种中的β-乳酸酶基因.
- 为了评估这些基因所赋予的抗生素耐药性.
- 为了确定这些抗性基因的可转移性.
主要方法:
- 选来自Akkermansia物种的八种代表性A类β-乳酸酶.
- 四种β-乳酸酶的净化和表征.
- 通过结构分析确定催化氨基酸 (51位的血清).
- 基因组上下文分析以评估基因可转移性.
主要成果:
- 阿克尔曼西亚β-乳酸酶增加了大肠杆菌对β-乳酸酶的最小抑制度 (2-1024倍).
- 四个纯化的β-乳酸酶被确定为扩展光谱,化青素,素和单菌剂.
- 位于51位的氨酸被验证为催化氨基酸.
- 基因组分析表明β-乳糖酶基因缺乏移动遗传元素,这表明可转移性较低.
结论:
- 阿克克曼西亚物种携带β-乳糖酶基因,使其对一系列β-乳糖抗生素产生抵抗力.
- 已识别的β-乳酸酶是功能扩展光谱酶.
- 遗传背景表明,这些耐药基因不易转移,支持Akkermansia作为益生菌的安全性.
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