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通过半理性设计使用糖酸化酶生产2-O-α-d-glucopyranosyl-l-ascorbic酸

Yujuan Shen¹, Jiajing Guo¹, Yuanyuan Xia¹

¹Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China; School of Biotechnology, Jiangnan University, Wuxi, China.

International journal of biological macromolecules

|December 1, 2024

概括

使用修改后的酸酸化酶,提高了2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G) 的酶合成. 该工程酶实现了358.6g/L的高产量,提高了健康和护肤应用的稳定性.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
生物化学生物化学

背景情况:

2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G) 在健康和护肤方面比l-ascorbic acid (L-AA) 提供了更好的稳定性.
使用糖酸酶 (SPase) 的酶合成是高产AA-2G生产的有希望的途径.

研究的目的:

通过设计一个Bifidobacterium longum SPase (BlSPase) 来增强AA-2G合成.
为了确定最佳条件和酶变体以改善AA-2G产量.

主要方法:

对BlSPase进行查和鉴定,其最佳pH值为5.4和温度为45°C.
使用分子建模,对接和突变发生 (氨酸扫描,和突变发生) 的BlSPase半理性设计.
创建和选一个突变库,确定L341V/V346P作为最佳变种.

主要成果:

鉴定了BlSPase在pH 5.4和45°C时具有最佳活性.
工程 BlSPase 变种 L341V/V346P 显示显著提高了性能.
在72小时内达到358.6g/L的高AA-2G产量,L-AA的摩尔转化率为75.7%.

结论:

半理性设计有效地改进了BlSPase,用于增强AA-2G合成.
这种工程酶和优化的条件代表了稳定的 Askorbic 酸衍生物生产的重大进步.
这种方法为商业应用生产AA-2G提供了可扩展和高效的方法.

关键词:

2-O-α-d-glucopyranosyl-l-ascorbic酸是什么意思? 2-O-α-d-glucopyranosyl-l-ascorbic酸是什么意思? 2-O-α-d-glucopyranosyl-l-ascorbic酸是什么意思? 2-O-α-d-glucopyranosyl-l-ascorbic酸是什么意思?生物转化生物转化突变的选择突变的选择一个半理性的设计.糖酸酸酶是一种糖酸酸酶.

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