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Updated: Jun 6, 2025

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

一种内在无序的RNA结合蛋白调节mRNA的翻译和存储

Xin Chen¹, Mashiat N Chowdhury², Hong Jin³

¹Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, United States.

Journal of molecular biology

|December 1, 2024

概括

像Sbp1这样的内在无序的RNA结合蛋白通过减缓翻译和促进mRNA存储来调节基因表达. 翻译后修改微调这些过程,指导mRNA进行蛋白质合成或分离.

科学领域:

分子生物学分子生物学
基因规则基因规则
蛋白质结构和功能蛋白质结构和功能

背景情况:

蛋白质中的内在无序区域 (IDR) 对于各种细胞功能至关重要,包括基因表达调节.
许多含有IDR的蛋白与细胞质核糖体相互作用,但它们的具体作用尚不清楚.
具有定义RNA识别动机和失序RGG域的RNA结合蛋白Sbp1作为研究IDR蛋白功能的模型.

研究的目的:

研究具有内在无序区域的RNA结合蛋白如何调节mRNA存储和翻译.
阐明Sbp1影响核糖体动力学和mRNA命运的分子机制.
了解翻译后修改在指导mRNA定位中的作用.

主要方法:

用基因组和分子方法研究Sbp1的功能.
电子显微镜被用来可视化多体结构.
进行了mRNA 5'UTRs和蛋白质相互作用体的分析.

主要成果:

Sbp1显著减缓了细胞mRNA上的核糖体运动,导致多体聚合和独特的环状结构.
与5'UTRs结合的Sbp1抑制了依赖和独立的翻译启动.
RGG动机的翻译后修改改变了Sbp1互动组,影响mRNA翻译与存储.

结论:

本质上有障碍的RNA结合蛋白积极促进多体聚合,并通过多种机制调节mRNA的翻译和储存.
Sbp1的功能突出显示了IDRs在控制基因表达中的关键作用.
这项研究为研究其他含有IDR的蛋白质提供了一个框架.

关键词:

这是一种RGG蛋白质.RRM RRM 是一个很好的方法.储存mRNA的时间在mRNA的翻译过程中,mRNA的翻译后翻译修改后的修改

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