了解牙周炎的发展过程中微生物群落结构的变化及其病变发生的情况
Junlin He1,2, Yefei Liu3,2, Hongzhen Xu4,2
1Department of Periodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China.
Clinical oral investigations
|December 1, 2024
概括
牙下微生物组的组成随着牙周炎的阶段和探测深度而显著改变. 早期预防至关重要,因为晚期牙周炎使恢复健康的口腔微生物组具有挑战性.
科学领域:
- 口腔微生物学 口腔微生物学
- 牙周病学 牙周病学
- 微生物组分析
背景情况:
- 牙周炎是一种复杂的口腔疾病,以牙周组织的炎症和破坏为特征.
- 牙下微生物群在牙周炎的发病和进展中发挥着关键作用.
- 了解与不同阶段和探测深度相关的微生物转移对于有效治疗至关重要.
研究的目的:
- 在中度牙周炎 (MP),重度牙周炎 (SP) 和牙周健康 (PH) 的受试者中描述牙下微生物群.
- 分析微生物群落结构在不同的探测深度 (PD):浅 (≤3毫米),中度 (4-6毫米) 和深 (≥7毫米).
- 调查与探测深度进展和牙周炎严重程度相关的微生物变化.
主要方法:
- 从50名受试者 (16名MP,17名SP,17名PH) 收集了下斑块样本.
- 根据牙周炎的阶段和PD,样品被分为六组:PHS,MPS,MPM,SPS,SPM和SPD.
- 使用高通量测序来分析收集的样品的微生物组成.
主要成果:
- 关于PD和牙周炎阶段 (p <0.05) 的牙下微生物群中观察到显著差异.
- 微生物多样性最初与PD增加,然后减少,而致病性属 (Fusobacterium,Filifactor,Porphyromonas) 增加和与健康相关的属 (Streptococcus,Haemophilus) 减少.
- 晚期牙周炎阶段 (SPM,SPD) 显示出明显的微生物群落,Treponema增加和Actinomyces减少,与中度阶段相比,更复杂,更有弹性的微生物共同网络.
结论:
- 下微生物组的组成与探测深度和牙周炎阶段密切相关.
- 在晚期牙周炎 (III/IV阶段) 中恢复健康的牙下微生物群可能很困难,这凸显了早期预防策略的重要性.
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