相关实验视频

Updated: Jun 6, 2025

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

调节真菌促进剂用于真核蛋白的表达

Charvi D Bandbe¹, Karan S Patil¹, Ejaj K Pathan²

¹Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Lavale, Pune, 412115, Maharashtra, India.

World journal of microbiology & biotechnology

|December 1, 2024

概括

菌类促进体是表达真核蛋白质的关键. 本综述详细介绍了它们的结构,功能和生物技术中的应用,有助于优化蛋白质生产.

科学领域:

生物技术和分子生物学
菌类学菌类学是指菌类学.

背景情况:

真菌系统 (酵母和线状真菌) 对于重组真核蛋白质生产是有效的.
优化蛋白质表达需要精确调节基因表达,由促进体驱动.
细丝真菌促进剂的表征并不是详尽的.

研究的目的:

提供关于真菌促进剂的全面审查.
讨论它们的结构,功能和影响因素.
探索未来的真菌促进剂的应用.

主要方法:

关于真菌促进物质的文献综述.
讨论促进器结构 (核心和近接元件).
根据功能对主办方进行分类.

主要成果:

菌类促进体是基因表达的关键 cis 调节元素.
讨论了来自不同真菌类的促进体的特性.
该审查涵盖了医疗保健,生物燃料,农业和生物技术中的促进器实用性.

结论:

了解真菌促进体对于开发高效的蛋白质表达平台至关重要.
量身定制的促进剂可以优化经济上重要的真核蛋白质的生产.
这些知识推动了真菌在各种生物技术应用中的应用.

关键词:

单核细胞蛋白表达的表达方式状真菌是一种状真菌.菌类促进剂菌类促进剂发起人工程发起人工程

更多相关视频

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Published on: October 4, 2017

相关概念视频

The Eukaryotic Promoter Region

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

Eukaryotic Transcription Inhibitors

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...