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校正:通过数字ELISA建立和描述noro-VLP测量

Takema Hasegawa1, Yuriko Adachi1, Kazumi Saikusa1

  • 1National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. hasegawa.takema@aist.go.jp.

Analytical methods : advancing methods and applications
|December 2, 2024
PubMed
概括

此更正澄清了用于定量诺罗病毒病毒样粒子 (VLP) 的数字ELISA方法. 它确保在分析方法中检测和表征诺罗病毒的准确数据.

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.