circEIF3I通过调节miR-328-3p/NCAPH轴促进结肠直肠癌转移
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Updated: Jun 6, 2025

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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
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Yali Zhao1, Yan He1, Zhiyuan Xiao2
1Department of Pathology, Shenzhen Longgang Central Hospital, Guangdong Province, Shenzhen, P. R. China.
Molecular carcinogenesis
|December 2, 2024
概括
循环RNA circEIF3I通过通过海绵miR-328-3p增加NCAPH表达来促进结直肠癌 (CRC) 转移. 针对这个circEIF3I/miR-328-3p/NCAPH轴为CRC提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 大肠直肠癌 (CRC) 是一种主要的胃肠道恶性瘤,具有显著的复发和转移率.
- 循环RNAs (circRNAs) 越来越被认为是癌症发展的关键参与者,但circEIF3I在CRC转移中的特定作用尚不清楚.
研究的目的:
- 为了研究circEIF3I在结直肠癌 (CRC) 转移中的作用.
- 阐明涉及miR-328-3p和NCAPH的潜在分子机制.
主要方法:
- 定量实时聚合酶链反应 (qRT-PCR) 用于基因表达分析.
- 细胞迁移和入侵的Transwell测试.
- 对于蛋白质表达和表皮质-介质细胞过渡 (EMT) 标记物的西部斑.
- 双露西法酶记者测定以确认分子相互作用.
- 异种移植瘤模型以评估体内转移.
主要成果:
- 在circEIF3I和NCAPH表达上调,而miR-328-3p在CRC组织和细胞下调.
- 击败circEIF3I抑制了CRC细胞的迁移,入侵,EMT和转移.
- circEIF3I作为miR-328-3p的分子海绵,导致NCAPH表达的增加.
- 过度表达NCAPH或抑制miR-328-3p可以逆转circEIF3I knockdown的抗转移作用.
结论:
- 环EIF3I/miR-328-3p/NCAPH轴通过上调NCAPH促进CRC转移.
- 这一轴代表了结直肠癌治疗的潜在新型治疗标.
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