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在怀孕中期和末期的人类先天性隔膜患者中,Yes相关蛋白质的调节失调

Yuichiro Miyake^1,2, Marietta Jank^1,3, Daywin Patel¹

¹Division of Pediatric Surgery, Department of Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba and Children's Hospital Research Institute of Manitoba, AE402-820 Sherbrook Street, Winnipeg, MB, R3A 1S1, Canada.

Pediatric surgery international

|December 2, 2024

概括

在先天性隔膜 (CDH) 中,与Yes相关的蛋白质 (YAP) 主要不活跃 (酸化YAP或p-YAP). 这种YAP失调可能会扰乱胎儿肺部的发育和分化.

科学领域:

发展生物学发展生物学
细胞信号传递细胞信号传递
医学研究医学研究

背景情况:

kongenital diaphragmatic hernia (CDH) 是一种严重的出生缺陷,影响肺部发育.
已知Yes相关蛋白 (YAP) 途径在细胞生长和器官生成中发挥作用.
在CDH的发病过程中,怀疑YAP失调.

研究的目的:

为了研究YAP的水平及其非活性形式,化YAP (p-YAP),在CDH病例的胎儿人类肺组织中.
为了比较YAP和p-YAP在CDH中的丰富度与妊娠中期和末期的控制胎儿肺部.
评估YAP对肺细胞分化标记物,HOPX和SPC的影响.

主要方法:

免疫光染色被用于量化胎儿肺组织中的YAP和p-YAP蛋白水平.
从具有CDH和对照的人类胎儿获得肺组织.
评估了分化标志物的表达,包括家庭主体单独蛋白X (HOPX) 和表面活性蛋白C (SPC).

主要成果:

与对照组相比,胎儿CDH肺组织显示YAP和p-YAP的丰度增加.
不活跃的形式,p-YAP,在CDH肺部显著升高,表明YAP失活.
在妊娠末期的CDH肺部,表现出更多的表面活性蛋白C阳性细胞和更少的家庭主体蛋白X阳性细胞.

结论:

在胎儿CDH肺中,YAP主要以非活性酸化形式 (p-YAP) 存在.
这种YAP失调可能会损害CDH中的正常肺发育和细胞分化.
YAP路径的改变可能与CDH的潜在机制有关.

关键词:

生产性腹膜带 Hernia 是一种先天性腹膜.河马信号通道的信号通道肺部低成形肺部低成形是的 - 相关蛋白质.

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