系统代谢工程用于增强Escherichia coli中的cytidine 5'-单酸盐生产
Tiantian Dong1, Wei Shen2, Yuanyuan Xia2
1School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
Journal of agricultural and food chemistry
|December 2, 2024
概括
工程化大肠杆菌菌株显著提高了丁5'-单酸盐 (5'-CMP) 的产量,这是食品和药品的重要化合物. 这项研究实现了417.9mg/L的创纪录标位,为可持续的工业制造铺平了道路.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物发酵 微生物发酵
背景情况:
- 丁5'-单酸盐 (5'-CMP) 是核酸衍生物的关键中间体.
- 5'-CMP在食品和制药行业得到了广泛的应用.
研究的目的:
- 为了改造大肠杆菌以提高5'-CMP的生产.
- 优化代谢途径以提高5'-CMP的产量和纯度.
主要方法:
- 大肠杆菌的基因改造以阻止5'-CMP降解途径.
- 5'-CMP二酸盐酸酶基因 (nudG) 的综合表达.
- 增强前体供应,特别是5--α-D-核糖1-二酸盐 (PRPP).
主要成果:
- 与野生型MG1655.5相比,工程化大肠杆菌CM006的5'-CMP标位增加了134.7倍.
- nudG的表达增强了5'-CMP标位,减少了耳酸,并减轻了反抑制.
- 大肠杆菌CM011通过阻断cytidine降解,达到338.2 mg/L的5'-CMP.
- 通过提高PRPP供应,大肠杆菌CM012通过提高PRPP供应实现了创纪录的417.9mg/L5'-CMP.
结论:
- 该研究表明,5'-CMP.在微生物生产中的显著进步.
- 开发的方法为工业5'-CMP制造提供了可持续和高效的方法.
更多相关视频
10:41The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
Published on: January 13, 2013
18.5K
09:45Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology
Published on: February 25, 2019
34.6K
相关概念视频
01:24Biosynthesis in Bacteria
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
01:24Other Glycolytic Pathways
1
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
1