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Updated: Jun 6, 2025

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
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A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

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探测深度聚硫化物波动在vivo通过工程可激活的光报道器与第二近红外窗口发射的发射

Lixin Sun1, Zhecha Zheng1, Ziwen Zhang2

  • 1Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, P. R. China.

Analytical chemistry
|December 2, 2024
PubMed
概括

研究人员开发了一种新的近红外II (NIR-II) 光探针,专门检测硫化 (H2S) 超过硫化 (H2S). 这一进步使得涉及H2S的生物过程能够得到更深入,更准确的成像.

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相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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