豆NCR13二硫化物交联变体在抗真菌活性和作用模式上表现出深刻的差异
James Godwin1, Arnaud Thierry Djami-Tchatchou1, Siva L S Velivelli1
1Donald Danforth Plant Science Center, St. Louis, Missouri, United States of America.
PLoS pathogens
|December 2, 2024
概括
小抗真菌NCR13中的二硫化键配对显著影响其效能和作用方式. 这种结构洞察力有助于设计农业新型生物真菌杀菌剂.
科学领域:
- 植物生物化学 植物生物化学
- 抗微生物研究的研究.
- 农业科学 农业科学
背景情况:
- 具有多种作用模式 (MoA) 的富含氨酸的小胺作为生物真菌杀菌剂具有前景.
- 豆类结节特定的富含氨酸 (NCR) 可以调节固定的细菌的分化.
- 一些阴离子NCR具有抗真菌活性,但二硫化物配对效应尚不清楚.
研究的目的:
- 调查二硫化物配对对豆NCR13.的抗真菌活性和MOA的影响.
- 阐明NCR13变体之间的不同抗真菌功效的结构基础.
主要方法:
- 在Pichia pastoris中表达豆NCR13,产生明显的折叠变体 (PFV1,PFV2).
- 核磁共振 (NMR) 用于结构确定PFV1和PFV2.
- 在体外测试以评估抗真菌功效,细胞透性,稳定性,核糖体RNA结合和蛋白质翻译抑制.
- 在植物中,对胡和番茄植物进行针对Botrytis cinerea的疗效测试.
主要成果:
- 产生了两个NCR13折叠变体 (PFV1,PFV2) 具有相同的序列但不同的二硫化物配对.
- 与PFV2.2相比,PFV1对多种植物病原体表现出更高的抗真菌活性.
- PFV1 显示增强了细胞透性,细胞内稳定性,核糖体RNA 结合和蛋白质翻译抑制.
- 在胡和番茄植物上,PFV1显著降低了灰菌病的症状.
结论:
- 硫化物键的配置极大地影响了NCR13.的抗真菌活性和MoA.
- PFV1和PFV2之间的结构差异是它们不同抗真菌功能的基础.
- 这些发现为开发用于农业的强效基生物杀菌剂提供了结构性基础.
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