染色体突变优于等离子体编码的突变,因为等离子体适应性成本补偿
Rosanna C T Wright1, A Jamie Wood2,3, Michael J Bottery1
1Division of Evolution, Infection and Genomic Sciences, University of Manchester, Manchester, United Kingdom.
PLoS biology
|December 2, 2024
概括
细菌染色体 (chrCM) 上的补偿突变 (CM) 在传播耐性方面优于等离子体 (plaCM) 上的突变. 这表明,chrCM细菌可能成为微生物群落中等离子体积累和传播的枢纽.
科学领域:
- 微生物生态学 微生物生态学
- 进化生物学 进化生物学
- 遗传学 是一个遗传学.
背景情况:
- 等离子体在微生物中促进横向基因转移,但会带来健康成本.
- 补偿突变 (CMs) 可以在质粒 (plaCM) 或染色体 (chrCM) 上出现,以减轻这些成本.
- 对于plaCM与chrCM对等离子体传播的生态影响尚不清楚.
研究的目的:
- 通过实验比较plaCM和chrCM的生态效应.
- 为了研究Pseudomonas fluorescens中等离子体补偿的进化动态.
- 了解驱动抗性巨质体传播的机制.
主要方法:
- 开发了一种新的实验系统,使用Pseudomonas光体SBW25和大质粒pQBR57.
- 进行了进化实验,以生成和研究plaCM和chrCM.
- 采用数学建模和分析方法来分析生态动态.
主要成果:
- 染色体CMs (chrCM) 在实验进化中占据主导地位,而不是等离子体CMs (plaCM),即使接受者数量众多.
- 在传输昂贵的等离子体 ("武器化") 方面,chrCM表现出更有效的补偿和优势.
- 一个涉及与水平传输的权衡的石纸剪刀 (RPS) 制度解释了plaCM无法占据主导地位的原因.
结论:
- 在促进等离子体传播和细菌适应方面,chrCM优于plaCM.
- 补偿细菌可以充当微生物群落中等离子体积累和传播的"枢纽".
- 了解CMs对于预测抗生素和耐药性基因的传播至关重要.
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