骨质细胞对长非编码RNAs的表观遗传调节的影响在骨质细胞中,这些骨质细胞在用纳米拓学培养在上
Rayana Longo Bighetti-Trevisan1, Luciana Oliveira Almeida2, Jaqueline Isadora Reis Ramos3
1Bone Research Lab, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, SP, Brazil; Faculty of Dentistry, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
Biomaterials advances
|December 2, 2024
概括
植入物的纳米拓学影响了骨细胞中的长非编码RNA (lncRNA) 表达,影响了骨质整合. 该lncRNA Kcnq1ot1直接针对Bglap mRNA,增强骨质细胞分化和植入物集成.
科学领域:
- 生物材料科学 生物材料科学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- (Ti) 植入物的骨质整合依赖于骨细胞通信,受长非编码RNA (lncRNAs) 等表观遗传因素的影响.
- 在Ti表面上的纳米拓 (Ti Nano) 促进骨质细胞分化,但可以通过表观遗传途径被骨质细胞抑制.
研究的目的:
- 调查骨质细胞调节在Ti Nano表面培养的骨质细胞中的IncRNA表达的假设.
- 阐明特定的lncRNAs的作用,如Kcnq1ot1,在纳米地形Ti上的骨质细胞和骨质细胞之间交叉的作用.
主要方法:
- 骨质细胞在Ti Nano和Ti Control表面培养,并与骨质细胞共同培养.
- 使用RNA测序 (RNAseq) 来识别差异表达的lncRNAs.
- 用RNA拉下测试和基因沉默来验证相互作用和功能角色.
主要成果:
- RNAseq确定了252个调节的lncRNAs,主要是在Ti纳米培养的骨质母细胞中.
- 在Ti Nano条件下发现Kcnq1ot1和特定的骨质原生标记mRNA (Alpl,Bglap,Bmp8a,Col1a1,Vim) 与骨质细胞之间存在负相关性.
- Kcnq1ot1直接针对BglapmRNA,在Ti纳米表面进行增强的相互作用,导致更大的骨质细胞抑制.
结论:
- 纳米结构的Ti表面调节骨质细胞-骨质细胞交叉,部分是通过调节骨质细胞中的IncRNA表达.
- lncRNA Kcnq1ot1通过与Bglap mRNA相互作用,在骨质细胞分化中发挥关键作用,这种相互作用受到纳米拓学和骨质细胞的影响.
- 这些发现为纳米拓学的骨质生成潜力提供了分子洞察力,支持改进的牙和骨假体的开发.
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