青春期激素通过法定和非法定途径在豆虫Riptortus pedestris中调节生殖隔膜
Abdul Hafeez1, Kou Wang1, Wen Liu2
1Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
青春激素 (JH) 通过Met-Kr-h1通路控制昆虫卵巢的发育. 然而,JH通过一个单独的,非正规的途径在Riptortus pedestris. diapause期间调节脂质积累.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 内分泌学 在内分泌学.
- 发展生物学 发展生物学
背景情况:
- 隔离是昆虫生存季节变化的关键适应,由荷尔蒙线索调节.
- 青春激素 (JH) 缺失传统上与生殖性隔膜特征 (如卵巢停产和脂质积累) 有关.
- 甲烯耐受性 (Met) 受体和克鲁佩尔同源1 (Kr-h1) 转录因子被认为是JH作用的主要媒介.
研究的目的:
- 研究青春激素 (JH) 在调节卵巢发育和生殖性隔离期间的脂质积累中的独特作用.
- 阐明特定的途径,包括正规的Met-Kr-h1途径,涉及JH介导豆虫Riptortus pedestris的隔膜特征.
主要方法:
- 在不同的光周期条件下 (长日与短日) 操纵Riptortus pedestris中的JH水平和作用.
- 使用JH模仿 (methoprene) 和基因沉默技术 (RNA干扰) 针对JH生物合成和信号基因 (Met, Kr-h1).
- 评估关键的隔离间隔指标:卵巢发育,脂质积累和生殖活动.
主要成果:
- 通过Met-Kr-h1通路,JH积极调节卵巢发育.
- JH独立地控制脂质积累,绕过Met-Kr-h1通路.
- 扰乱Met或Kr-h1影响了卵巢发育,但没有JH诱导的脂质减少.
结论:
- 青春期激素 (JH) 使用正规的Met-Kr-h1通路来调节卵巢在隔膜期的发育.
- JH采用一种替代的,非正规的途径来控制脂质积累,独立于Met和Kr-h1.
- 这一发现揭示了JH在昆虫尿路生理学中的双重作用机制.
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