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开发一种基于酵母和细菌表面显示技术的先进的乙甲检测溶液

Weigeng Liu¹, Jiamin Cao¹, Di Wu¹

¹College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.

Journal of biotechnology

|December 2, 2024

概括

这项研究开发了一种全细胞光学生物传感器,用于检测饮料中发现的致癌物乙化物. 生物传感器利用酵母和大肠杆菌表面显示的乙甲脱酶 (AldH),提供了灵敏有效的检测方法.

科学领域:

生物技术是生物技术.
生物传感器开发开发
环境监测环境监测

背景情况:

乙甲是饮料和环境中普遍存在的致癌物.
有效和方便的乙甲检测方法至关重要.
目前的检测方法可能缺乏灵敏度或方便性.

研究的目的:

开发一种灵敏,方便和高效的全细胞光学生物传感器,用于检测乙甲.
为了设计Saccharomyces cerevisiae和大肠杆菌以显示乙甲脱酶 (AldH) 的表面.
优化和比较酵母和细菌生物传感器平台的性能.

主要方法:

在使用花素的Saccharomyces cerevisiae和在使用冰核蛋白 (INP) 的大肠杆菌上显示乙甲脱酶 (AldH) 的表面显示.
使用激光扫描共聚焦显微镜确认表面显示.
反应条件 (温度,pH) 的优化和显示系统的比较.
开发的生物传感器在葡萄酒和饮料样本中检测乙甲的应用.

主要成果:

在酵母和大肠杆菌上成功构建并确认了AldH的表面显示.
确定酵母 (3.68 ± 0.07 U/mL/OD600) 和大肠杆菌 (6.95 ± 0.04 U/mL/OD600) 的最佳反应条件和最高全细胞活性.
在真实样本中检测乙甲的表现出基质特异性和准确性,回收率从94.4%到110.1%.

结论:

工程酵母和大肠杆菌菌株显示AldH功能作为有效的全细胞光学生物传感器对乙.
开发的生物传感器提供了一种灵敏,方便和高效的方法来检测饮料中的乙甲,包括红酒.
这种方法有望监测食品和环境样本中的乙甲水平.

关键词:

乙甲基脱基酶的使用乙甲检测检测方法细胞表面显示器显示器美国大肠杆菌 (E. coli).这种植物是Saccharomyces cerevisiae.

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