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Updated: Jun 6, 2025

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
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高分辨率的向质谱仪用于全面量化脂质:临床应用和细胞外囊泡的表征

Thiago V D Felippe1, Diana M Toro2, Jonatan C S de Carvalho3

  • 1Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto - FFCLRP, Universidade de São Paulo-USP, Ribeirão Preto, 14040-901, SP, Brazil.

Analytical biochemistry
|December 2, 2024
PubMed
概括

这项研究提出了一种新的液体染色学-并联质谱法方法,用于精确量化脂. 该方法在复杂的生物样本中准确识别脂类物种,有助于发现生物标志物.

关键词:
在 COVID-19 疫情中,细胞外囊泡中的细胞外囊泡.这是LC-MS/MS.斯芬戈脂类是指状的脂类.

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High-Resolution Mass Spectrometry (HRMS)01:15

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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