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基于生物薄膜的工程化Komagataella phaffii的固定发酵,用于生产西兰酶

Huanqing Niu¹, Daoguang Zhu², Jing Leng²

¹State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, China.

Bioresource technology

|December 2, 2024

概括

具有增强生物膜形成的工程化Komagataella phaffii增强了连续的西兰酶生产. 过度表达PAS_FragB_0067提高了35.4%的酶活性和固定发酵中的细胞粘附.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
酶的生产酶的生产

背景情况:

科马加塔 (Komagataella phaffii) 是一种用于工业酶生产的酵母.
固定发酵可以提高微生物生产效率.
改善K. phaffii中的生物膜形成可以优化固定发酵.

研究的目的:

为了改造Komagataella phaffii,以改善生物膜的形成.
开发一种不动化的发酵工艺,用于连续的西兰酶生产.
阐明K. phaffii.生物基发酵的分子机制.

主要方法:

对K. phaffii进行基因工程,以过度表达与生物膜相关的基因 (PAS_chr2-2_0178和PAS_FragB_0067).
基于生物膜的固定发酵系统的开发.
酶活性测定和转录组分析.

主要成果:

过度表达PAS_chr2-2_0178和PAS_FragB_0067的生物膜形成分别增加了31.6%和113.8%.
与对照组相比,PAS_FragB_0067-过度表达菌株在五个批次中实现了35.4%的平均西兰酶活性.
增强生物膜形成改善了细胞粘附性,并为发酵提供了更多活跃的细胞.
转录组分析表明,生物膜基因过度表达促进了中央碳代谢.

结论:

过度表达PAS_FragB_0067是一种可行的策略,可以增强固定K. phaffii.的生物膜形成和西兰酶生产.
基于生物膜的固定发酵为高效的连续酶生产提供了一个有前途的方法.
这项研究提供了通过基因工程优化K. phaffii发酵的分子见解.

关键词:

生物膜的形成过程.固定化的发酵过程科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.对转录组进行分析.在Xylanase的使用中.

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