不同的高速反流色谱注射模式的比较,用于分离格拉布里丁
Sheng-Bing Wang1,2, Kun Yang1,2, Xing-Cui Wang2
1College of Science, Gansu Agricultural University, Lanzhou, China.
Journal of separation science
|December 3, 2024
概括
在逆流色谱中,连续注射模式显著提高了分离效率,并减少了将glabridin从Glycyrrhiza glabra中分离的溶剂消耗. 这种方法对于天然产品的净化非常有效.
科学领域:
- 自然产品化学 自然产品化学
- 染色学分离科学 染色学分离科学
背景情况:
- 甘 (Glycyrrhiza glabra) 是一种有价值的生物活性化合物的来源.
- 格拉布里丁是甘中一种关键的异黄,具有显著的药理特性.
- 对格拉布里丁的有效隔离和净化对其应用至关重要.
研究的目的:
- 为了比较反流色谱的不同注射模式,以隔离格拉布里丁.
- 确定最有效的方法来从Glycyrrhiza glabra.净化格拉布里丁.
- 评估注射模式对分离效率,时间和溶剂消耗的影响.
主要方法:
- 使用反流色谱 (CCC) 采用单次,重叠和连续注射模式.
- 使用双相溶剂系统:n-hexane/乙烯/甲醇/水 (5:4:5:4,v/v).
- 在注入模式之间比较分离效率,时间,溶剂消耗,产量,纯度和回收率.
主要成果:
- 与单次注射相比,连续注射模式显示出明显更高的分离效率.
- 连续注射将总分离时间减少到53.97%,溶剂消耗减少到单次注射模式的52.49%.
- 叠加注射也提高了效率,将时间减少到89.68%,将溶剂消耗减少到单次注射的74.88%.
- 在所有测试的注射模式中,产量,纯度和回收率都是可比的.
结论:
- 连续注射模式下的高速反流色谱 (HSCCC) 是用于隔离格拉布里丁的最有效方法.
- 连续注入为天然产品净化提供了卓越的效率和资源节约.
- 这种优化的CCC方法具有净化其他有价值的天然产品的潜力.
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