用生物信息学分析识别乳腺癌中hsa_circ_0001314相关的ceRNA网络
Yuge Ma1, Bing Zhang1, Liping Wang1
1Department of Pathology, Qiqihar Medical University, Qiqihar, China.
International journal of genomics
|December 3, 2024
概括
循环RNA hsa_circ_0001314 在乳腺癌 (BC) 中被上调. 它的淘汰抑制BC细胞的增殖和入侵,这表明它是一个潜在的治疗目标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 正在成为癌症的关键调节者,包括乳腺癌 (BC).
- 在BC病变发生过程中对circRNAs的研究仍在发展,这凸显了识别新型参与者的必要性.
- 通过数据库分析,hsa_circ_0001314被确定为BC的潜在显著circRNA.
研究的目的:
- 研究hsa_circ_0001314在乳腺癌中的作用和机制.
- 要确定hsa_circ_0001314是否可以作为BC的预后生物标志物或治疗点.
主要方法:
- 对GEO数据库数据集的生物信息分析,以识别BC中上调的circRNAs.
- 用RT-qPCR验证BC组织和细胞中的hsa_circ_0001314表达.
- 功能测试 (CCK-8,伤口愈合,穿孔入侵,流动细胞计) 来评估hsa_circ_0001314对BC细胞的冲击影响.
- 生物信息学和RT-qPCR用于预测和确认与微RNA (miRNA) 和下游信号通路的相互作用.
主要成果:
- hsa_circ_0001314在乳腺癌组织和细胞中显著上调.
- 镇压hsa_circ_0001314抑制了BC细胞的增殖,迁移和入侵,同时促进了细胞亡.
- hsa_circ_0001314作为hsa-miR-548aj-3p的分子海绵,通过MAPK8和MAP3K1.1激活MAPK信号通路.
结论:
- hsa_circ_0001314在促进乳腺癌进展方面发挥着至关重要的作用.
- hsa_circ_0001314通过海绵化hsa-miR-548aj-3p作为ceRNA的功能,影响MAPK通路.
- hsa_circ_0001314作为一种新的预后生物标志物和乳腺癌的治疗点具有前途.
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