相关实验视频

Updated: Jun 6, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

ACE2,从脏到SARS-CoV-2:唐纳德·塞尔丁2023年颁奖演讲

Daniel Batlle¹, Luise Hassler¹, Jan Wysocki¹

¹Division of Nephrology/Hypertension, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL.

Hypertension (Dallas, Tex. : 1979)

|December 3, 2024

概括

可溶性 ангиотензин转化酶2 (ACE2) 蛋白质通过向脏宁- ангиотензин系统和中和SARS-CoV-2提供双重治疗潜力. 生物工程可溶性ACE2提供了对SARS-CoV-2变种的保护.

科学领域:

生物化学生物化学
分子生物学分子生物学
病毒学病毒学

背景情况:

ангиотензин转化酶2 (ACE2) 是一种关键的酶,可以代谢 ангиотензин II (Ang II),并作为 SARS-CoV-2 的细胞受体.
膜结合的ACE2具有脏保护作用,而可溶性ACE2可能具有治疗效益.
ACE2的双重作用为心血管和传染病提供了一个独特的治疗点.

研究的目的:

开发可溶性ACE2截断剂,针对脏宁-血管素系统.
设计一种具有增强SARS-CoV-2结合亲和力和长时间作用的可溶性ACE2蛋白.
为了评估生物工程可溶性ACE2对SARS-CoV-2感染的治疗疗效.

主要方法:

针对脏的ACE2截断器的设计.
一种可溶性ACE2蛋白 (ACE2 618-DDC-ABD) 的生物工程,具有改进的SARS-CoV-2结合.
在感染SARS-CoV-2变种的k18hACE2小鼠中注射ACE2 618-DDC-ABD.

主要成果:

工程化可溶性ACE2 (ACE2 618-DDC-ABD) 显示了对SARS-CoV-2的结合亲和力增加.
在感染SARS-CoV-2的小鼠中,鼻内注射提供了几乎完全的抗致死性保护.
可溶性ACE2蛋白显示出立即作用和针对多种SARS-CoV-2变异的广泛疗效.

结论:

可溶性ACE2蛋白是SARS-CoV-2中和的有效治疗剂.
基于ACE2的疗法为当前和新兴的SARS-CoV-2变种提供了通用方法.
准ACE2对治疗脏疾病和病毒感染有很大的希望.

关键词:

在 COVID-19 疫情中,这就是SARS-CoV-2病毒.ангиотензин II 的使用.血管氨酸转化酶 2 2 病毒内部化病毒内部化

更多相关视频

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Published on: January 8, 2018

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

相关概念视频

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...