通过外体miR-9-3p向NQO2来调节线粒体功能,FRK具有致癌作用
Hong Huang1,2, Yan Wang3, Jiadi Gan4
1Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
概括
与Fyn相关的激酶 (FRK) 促进非小细胞肺癌 (NSCLC) 的进展. 携带miR-9-3p的外体通过准NQO2来调解这种情况,影响线粒体功能和癌症代谢.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 与Fyn相关的激酶 (FRK) 与非小细胞肺癌 (NSCLC) 的进展和代谢有关.
- 精确的分子机制,特别是外体的作用,仍然需要完全阐明.
研究的目的:
- 研究外体在FRK介导NSCLC进展中的作用.
- 为了确定关键的分子介质,如microRNAs,参与这个过程.
- 探索下游目标和对癌细胞代谢和线粒体功能的功能后果.
主要方法:
- 从FRK-Knockout (KO) 和控制NSCLC细胞中分离和共培养外体细胞.
- 微RNA (miRNA) 测序以识别外体miRNAs.
- 在共同培养系统中应用miRNA和外基因组抑制剂 (GW4869).
- 评估细胞增殖,转移,新陈代谢和线粒体功能.
- 对潜在目标基因NQO2表达和功能的分析.
主要成果:
- 来自FRK-KO细胞的外体对NSCLC细胞增殖,转移,新陈代谢和线粒体功能产生了负面影响.
- 异位体miR-9-3p被确定为一个关键的媒介.
- 抑制miR-9-3p或外体释放减弱了恶性表型和线粒体功能障碍.
- NQO2被确定为外体miR-9-3p的潜在标,它参与调节线粒体功能.
结论:
- FRK通过外体miR-9-3p促进NSCLC恶性进展和代谢重编程.
- 这一途径涉及准NQO2以增强线粒体功能.
- 外体miR-9-3p代表了NSCLC的一个新的治疗点.
更多相关视频
08:49Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
Published on: May 27, 2016
11.1K
09:40Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
2.6K
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:46Electron Transport Chain: Complex I and II
11.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.5K
03:03mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
02:38Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
02:23Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
02:55The Nucleolus
8.7K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.7K