使用DNA彗星测试揭示MXenes的潜在基因毒性
Sergiy Kyrylenko1, Inna Chorna1, Zhanna Klishchova1,2
1Biomedical Research Center, Sumy State University, 31 Sanatorna Street, Sumy 40007, Ukraine.
ACS applied bio materials
|December 3, 2024
概括
像Ti3C2Tx和Nb4C3Tx这样的亚微米MXene片可以在活细胞中引起DNA损伤,但没有细胞毒性. 这种基因毒性影响与片大小和电场暴露有关,而不是固有的材料特性.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
背景情况:
- MXenes是多功能2D材料,在科学和技术中具有广泛的应用,包括有前途的生物医学用途.
- 尽管证明了生物相容性和低细胞毒性,但MXenes的基因毒性在很大程度上仍未得到研究.
- 潜在的应用包括光热疗法,药物输送,生物传感和抗菌涂层.
研究的目的:
- 通过检查它们对培养细胞中DNA完整性的影响来研究MXenes的基因毒性.
- 确定MXenes是否干扰染色体DNA,并评估材料特性和细胞活性的作用.
- 为了阐明任何观察到的DNA损伤背后的机制.
主要方法:
- 培养的小鼠黑色素瘤和人类纤维细胞被载入了Ti3C2Tx和Nb4C3Tx MXenes.
- 使用DNA彗星测试检测染色体DNA碎片化.
- 评估了MXene薄片大小,细胞活力和实验条件 (例如电泳) 的影响.
主要成果:
- MXenes (Ti3C2Tx和Nb4C3Tx) 诱导了DNA彗星,表明活细胞中的基因毒性.
- 亚微米MXene片对于诱导DNA彗星至关重要,而较大的片没有.
- 没有观察到细胞毒性,并且在死细胞或纯化DNA中没有发生DNA碎片.
- 拟议的机制涉及微米MXene片的运动和旋转在电场下的细胞内,导致DNA碎片.
结论:
- 亚微米MXene颗粒可以透到活细胞中,并诱导DNA碎片化,特别是在电场下,这表明了尺寸依赖的基因毒性机制.
- MXenes表现出极好的生物相容性和低细胞毒性,基因毒性取决于颗粒大小和细胞环境.
- 这些发现对于开发安全的基于MXene的生物医学应用至关重要,特别是在癌症治疗中.
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