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FMRFamide G蛋白结合受体 (GPCR) 在西鱼中:鉴定,特征和表达特征

Jian-Jun Xie¹, Ying Li¹, Jun-Hong Wu¹

¹National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, China.

|December 3, 2024

概括

研究人员克隆了来自日本菌的FMRFamide G蛋白结合受体 (SjFaGPCR). 这种受体在关键的大脑和视叶区域表达,这表明它在生殖调节中的作用.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

FMRFamide是一种关键的神经调节剂,参与动物王国的各种生理过程.
G蛋白结合受体 (GPCRs) 调解细胞对神经结合的反应,影响各种功能.
之前的研究表明,FMRFamide参与调节日本菌的性成熟.

研究的目的:

克隆来自日本菌的FMRFamide G蛋白结合受体 (SjFaGPCR) 的全长cDNA.
研究SjFaGPCR在S. japonica中的表达模式和潜在相互作用.
提供关于FMRFamide信号在生殖调节中的作用的分子洞察力.

主要方法:

完整的cDNA克隆SjFaGPCR的全长.
使用同质模型进行3D结构预测.
多个序列对齐和遗传学分析.
在现场杂交 (ISH) 用于组织表达分析.
定量实时PCR (qRT-PCR) 用于基因表达造型.
在HEK293细胞中进行同局部化研究.

主要成果:

克隆的SjFaGPCR基因长1396bp,编码了一个418氨基酸蛋白质,具有7个跨膜区域.
遗传学分析显示,SjFaGPCR在无脊椎动物,特别是头足动物中得到保护.
ISH检测到SjFaGPCR信号在视叶和大脑区域 (食道上和食道下群体).
qRT-PCR显示,在特定的繁殖阶段,雄性和雌性的大脑和视叶中SjFaGPCRmRNA表达更高.
同定位实验表明SjFMRFamide和SjFaGPCR之间的潜在相互作用.

结论:

这项研究成功地克隆和表征了来自日本菌的SjFaGPCR.
表达模式表明SjFaGPCR在中枢神经系统中起着重要作用,特别是在大脑和视中.
这些发现支持了通过SjFaGPCR传递FMRFamide信号的假设,即FMRFamide通过SjFaGPCR参与S. japonica的生殖调节.

关键词:

生物信息学分析表达方式表达方式表达在FMRFamide GPCR中使用.互动互动互动互动日本虫 (Sepiella japonica) 是一种日本虫.

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