相关实验视频

Updated: May 5, 2026

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

基于计算机模拟和实验验证:从土壤微生物群中挖掘和表征新型抗菌

Chunming Xu¹, Aiping Han², Yuan Tian²

¹School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China; Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China.

|December 3, 2024

概括

我们开发了一个深度学习管道,从土壤DNA中找到抗微生物 (AMP). 这种方法有效地确定了食品安全应用的有希望的AMP候选者.

科学领域:

微生物学微生物学
生物信息学是一种生物信息学.
食品科学食品科学食品科学

背景情况:

传统的抗微生物 (AMP) 查复杂且昂贵.
增强营养方法对于改善食品安全和延长保质期至关重要.
需要新的方法来有效地从大型数据集中发现AMP.

研究的目的:

开发和验证深度学习管道,以从土壤元基因组数据中识别潜在的AMP.
发现新的AMP候选物,在食品保存中具有潜在的应用.
为了证明计算方法在加速AMP发现方面的有效性.

主要方法:

一个深度学习模型被训练并应用于土壤元基因组数据.
候选AMP使用表面电荷分析,Hemopred和ToxinPred进行了选.
用分子对接和动力学模拟来评估结合亲和力和稳定性.
为了验证,进行了化学合成和抗菌活性测试.

主要成果:

深度学习管道在AMP预测中实现了高准确度 (92.71%) 和精度 (91.29%).
确定了9种候选,其中P4 (GTAWRWHYRARS) 显示出与MrkH.最好的结合亲和力.
合成的P4对Klebsiella pneumoniae,大肠杆菌和金黄色葡萄球菌表现出显著的抗菌活性.

结论:

开发的深度学习管道有效地发现了从元基因组数据中发现新的AMP.
P4显示出作为食品应用中安全有效的抗微生物剂的潜力.
这种综合方法加速了AMP的发现,为传统方法提供了一个有希望的替代方案.

关键词:

生物活性酸是一种生物活性酸.食品生物防腐剂食品生物防腐剂高吞吐率的高吞吐量.机器学习机器学习预测模型的预测模型.

更多相关视频

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Published on: February 14, 2018

Isolation of Soil Microorganisms Using iChip Technology

Isolation of Soil Microorganisms Using iChip Technology

Published on: January 10, 2025

相关概念视频

Soil Microbial Ecology

Soil Microbial Ecology

Soil microbial ecology is defined by highly diverse, spatially structured communities that drive nutrient cycling, organic matter turnover, and overall ecosystem stability. Although a gram of soil can contain thousands of bacterial and archaeal taxa, the ecological processes they mediate are even more crucial for sustaining terrestrial life.Microhabitats and NichesSoil is a heterogeneous mixture of minerals, organic matter, water, and air. Microbes inhabit distinct microhabitats formed by...

iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...