在中央代谢酶的进化过程中,自我组装的频繁过渡
Franziska L Sendker1, Tabea Schlotthauer1, Christopher-Nils Mais2
1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany.
Nature communications
|December 3, 2024
概括
蛋白酶组合可以在中性进化,而不仅仅是为了功能. 酸盐合成酶在整个进化过程中表现出各种结构,变化不会影响催化活性,这表明组装灵活性.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 结构生物学 结构生物学
背景情况:
- 同型蛋白质复合体很常见,通常认为结构决定功能.
- 蛋白质组装的进化驱动因素,例如功能优势与中性变异,仍在争论中.
- 从历史上来看,在大型蛋白质家族中测量四级结构变异一直是具有挑战性的.
研究的目的:
- 为了研究 prokaryotic 酸盐合成酶中 homo-oligomeric 组合的进化动态.
- 为了确定蛋白质组合的变化是否提供功能优势或受到中性漂移的影响.
- 探索酶结构,功能和进化史之间的关系.
主要方法:
- 利用质量摄影仪进行大规模的四分制结构测量.
- 运用了遗传学来分析进化关系.
- 纳入结构生物学技术,以获得详细的结构洞察力.
- 进行了体外和体内活体功能实验.
主要成果:
- 在 prokaryotic 中发现了各种各样的 homo-oligomeric 组装状态,在整个细胞系过程中合成酸盐.
- 在组装状态中观察到并行进化和逆转的实例.
- 发现组装类型之间的进化过渡不会显著改变酶催化效率.
- 证明了酶组合可以随着相当大的灵活性演变.
结论:
- 蛋白质组装状态可以以中性方式演变,独立于强大的功能约束.
- 酶可能会在进化时间内探索广泛的组装配置.
- 在整个族系中描述结构-功能关系对于理解进化过程至关重要.
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