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Updated: Jun 5, 2025

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采用多孔板方法,在组织清除过程中增加样品吞吐量

Fumito Akiyama1,2, Katsuhiko Matsumoto1,3, Katsunari Yamashita1,4

  • 1Laboratory for Synthetic Biology, Center for Biosystems Dynamics Research, RIKEN, Suita, Japan.

Nature protocols
|December 3, 2024
PubMed
概括

这项研究介绍了一种用于3D病理学的高通量方法,该方法使用在六井板中并行清除组织. 这种方法简化了样本处理,减少了生物医学诊断和研究的实践时间.

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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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