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单次长时间的压力会诱导中部前额叶皮质的行为和转录基因变化,从而增加大鼠对类似焦虑行为的易感性
Javed Iqbal1,2, Geng-Di Huang1, Dan Shen3
1Department of Addiction Medicine, Shenzhen Clinical Research Center for Mental Disorders, Shenzhen Kangning Hospital and Shenzhen Mental Health Center, Clinical College of Mental Health, Shenzhen University Health Science Center, Affiliated Mental Health Center, Southern University of Science and Technology, Shenzhen, China.
Frontiers in psychiatry
|December 4, 2024
概括
创伤性压力会改变中间前额叶皮层 (mPFC) 中的基因表达,影响与神经退行和类似成行为的途径. 临界前 (PL) 和临界下 (IL) 皮层中的这些转录组变化可能解释易感个体的焦虑类行为.
科学领域:
- 神经科学是一个神经科学.
- 基因组学就是基因组学.
- 精神病学是一个精神病学.
背景情况:
- 创伤性压力可能导致神经精神障碍,如PTSD.
- 中间前额皮层 (mPFC) 对于情绪调节和认知功能至关重要.
- 以前的研究表明,PTSD中mPFC功能发生改变,但具体的基因表达变化仍然不清楚.
研究的目的:
- 为了研究创伤性压力下的mPFC中转录组范围内的基因表达变化.
- 为了确定受影响在前边缘 (PL) 和下边缘 (IL) 皮层的特定分子通路.
- 了解压力后类似焦虑行为的转录基础.
主要方法:
- 利用单一长期压力 (SPS) 动物模型来诱导创伤性压力.
- 在PL和IL皮层上进行了全转录组RNA-seq分析.
- 使用开放场地和升高加迷宫测试评估类似焦虑的行为.
主要成果:
- 在PL和IL mPFC中鉴定了62个差异表达基因 (DEG).
- PL皮层 DEGs 在突触和细胞骨功能中的丰富性降低.
- IL皮层DEGs表明神经元发育和学习途径的丰富性减少.
- 在神经退行性疾病的KEGG途径中,PL皮层的DEGs被丰富.
- IL皮层DEGs在与压力信号和类似成行为的途径中得到了丰富.
结论:
- 单次长期应激 (SPS) 在PL和IL mPFC中诱导出明显的转录基因变化.
- 这些分子路径的改变可能会导致差异性应对机制和焦虑类行为.
- 已识别的途径提供了关于压力诱导疾病病理生理学的见解.
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