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相关概念视频

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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What is Genetic Engineering?00:49

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Overview
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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相关实验视频

Updated: Jun 5, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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基于CRISPR的基因编辑技术及其在微生物工程中的应用.

Junwei Wei1, Yingjun Li1

  • 1State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Engineering microbiology
|December 4, 2024
PubMed
概括
此摘要是机器生成的。

像CRISPR-Cas这样的基因编辑技术可以实现精确的基因修改. 本综述涵盖了CRISPR-Cas系统,基编辑,主要编辑和CRISPR相关的转移酶技术,用于微生物中的应用.

关键词:
这就是CRISPR-Cas系统.基因编辑 基因编辑工业微生物 工业微生物益生菌 益生菌 益生菌

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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科学领域:

  • 分子生物学分子生物学
  • 基因工程是一种基因工程.
  • 生物技术是生物技术.

背景情况:

  • 基因编辑允许针对新功能或表型的DNA进行有针对性的修改.
  • 聚类正规间隔的短平行体重复 (CRISPR-Cas) 系统为细胞和生物提供了高效的基因工程工具.

研究的目的:

  • 为了回顾新兴的基因编辑工具:指核酶 (ZFNs),转录激活器样效应核酶 (TALENs) 和CRISPR-Cas系统.
  • 详细介绍CRISPR-Cas机制,策略 (内源,外源) 和新技术,如基编辑器 (BE),主要编辑器 (PE) 和CRISPR相关转基因酶 (CAST).
  • 总结基于CRISPR在工业微生物和益生菌中的应用,讨论未来的挑战和前景.

主要方法:

  • 对ZFN,TALEN和CRISPR-Cas技术进行了审查.
  • 详细解释了CRISPR-Cas系统的原理,分类和机制.
  • 基因编辑策略的描述,包括BE,PE和CAST技术.

主要成果:

  • 概述三个主要的基因编辑工具及其机制.
  • 详细了解基于CRISPR的高级编辑策略和新技术.
  • 在修改工业微生物和益生菌中使用CRISPR的概述.

结论:

  • 克里斯普尔-卡斯技术是基因工程的一个强大工具.
  • 像BE,PE和CAST这样的新兴技术扩大了基因编辑能力.
  • 尽管面临挑战,基于CRISPR的工具显示出工业生物技术的巨大潜力.