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识别和验证circDOCK1/miR-138-5p/GRB7促进乳腺癌进展的轴

Yan Zhang¹, Mei Yang^1,2, Yiping Wang¹

¹Department of Breast Surgery, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, 510630, People's Republic of China.

Breast cancer (Dove Medical Press)

|December 4, 2024

概括

一种新的循环RNA,circDOCK1,通过调节circDOCK1/miR-138-5p/GRB7轴,促进HER2阳性乳腺癌的转移和进展. 这一发现为乳腺癌治疗提供了潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

非编码RNA越来越多地被认为是它们在人类瘤中的作用.
RNA相互作用网络对于乳腺癌的发展至关重要.
循环RNAs (circRNAs) 是一种新型的非编码RNAs类,具有新兴意义.

研究的目的:

为了确定涉及乳腺癌的新型循环RNA.
阐明这些循环RNAs的生物机制.
探索它们作为乳腺癌治疗点的潜力.

主要方法:

HER2阳性乳腺癌组织和配对的正常组织的全转录组RNA测序.
差异表达的circRNAs,miRNAs和mRNAs的识别和网络构建.
生物信息分析,化酶测定和RIP测定用于验证.
定量逆转录PCR (qRT-PCR) 用于circDOCK1表达分析.
功能性救援实验,以评估circDOCK1/miR-138-5p/GRB7轴的作用.

主要成果:

鉴定了6960个差异表达的mRNA,133个miRNA和1691个circRNA.
丰富性分析表明它参与细胞分裂,细胞循环和DNA修复途径.
一个circDOCK1/miR-138-5p/GRB7竞争的内源RNA (ceRNA) 网络被构建和验证.
发现circDOCK1轴促进乳腺癌细胞迁移和入侵.
升高的circDOCK1表达与不良的临床病理特征和患者的不良结果相关.

结论:

一个新的circDOCK1/miR-138-5p/GRB7轴显著促进HER2阳性乳腺癌的转移和进展.
CircDOCK1代表了乳腺癌治疗的潜在治疗标.

关键词:

乳腺癌乳腺癌乳腺癌 ceRNARNA是什么意思一个圆形的DOCK1 患者的生存率患者的生存率这是一个渐进的过程.

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