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Updated: Jun 29, 2026

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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
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人口口腔伤口愈合的动力学和相关的微生物群
H Yuan1,2, G E Chlipala3, H I Bangash1,2
1Department of Periodontics, College of Dentistry, University of Illinois Chicago, Chicago, IL, USA.
Journal of dental research
|December 4, 2024
概括
这项研究引入了一种新的人类硬 palatus 伤口模型,揭示了更快的伤口关闭和在愈合过程中基因表达和口腔微生物群的显著变化. 该模型提供了对优质口腔伤口修复机制的见解.
科学领域:
- 口腔粘膜伤口愈合研究研究
- 人类组织模型.
- 微生物组的分析
背景情况:
- 与皮肤相比,口腔粘膜表现出优越的伤口愈合,其特点是快速关闭,减少炎症和最小的痕.
- 良好的人口口腔伤口模型对于研究愈合机制和评估临床干预措施至关重要.
- 现有的人类口腔伤口模型缺乏全面的表征.
研究的目的:
- 描述和表征一种新的人类硬 palates 伤口愈合模型.
- 调查口腔伤口愈合期间伤口关闭,基因表达,蛋白质水平和微生物群的动态变化.
- 为未来的口腔伤口研究和治疗开发提供一个强大的平台.
主要方法:
- 创建了标准化的全厚圆形和矩形伤口在人类的硬 palates.
- 监测伤口关闭,收集16SrRNA测序 (微生物组分析) 的拭子.
- 对基因表达和酶相关的免疫吸收试验进行实时聚合酶链反应,以检测伤口组织和唾液中的蛋白质水平.
主要成果:
- 圆形伤口在第7天几乎完全关闭.
- 在第1天和第3天观察到炎症性细胞因子,生长因子,抗微生物和细胞外矩阵分子的基因表达显著改变.
- 微生物组分析揭示了伤口表面细菌殖民的显著时间变化.
结论:
- 开发的人类硬 palatal 伤口模型是全面的特征.
- 该模型有效地捕捉了伤口关闭,介质表达和口腔微生物组变化的动态变化.
- 该模型作为一种有价值的工具,可以促进对口腔伤口愈合和测试干预措施的理解.
相关概念视频
Introduction to the Human Microbiota
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...
Development of Human Microbiota
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...
The Skin Microbiota
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Development of the Oral Microbiota
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
Microbiota of the Respiratory Tract
The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more like...

