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阿拉比多普西斯E3环型联酶TEAR4通过向RGA进行降解来控制种子发芽

Hao Yu¹, Yutao Wang¹, Yi Wang¹

¹State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|December 4, 2024

概括

结合1结合环型E3结合酶 (眼) 冗余地促进DELLA蛋白降解,这对于Arabidopsis中光调节的种子发芽至关重要. 这条古老的路径,在植物中得到保护,通过降低DELLAs来微调发芽.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

种子发芽对于植物生存和农业至关重要,受到光线和DELLA蛋白质的关键调节.
在发芽期间在不同的光照条件下,DELLA蛋白质降解的精确机制尚未完全理解.

研究的目的:

为了阐明TIE1-ASSOCIATED环型E3结合酶 (TEARs) 在DELLA蛋白质降解和光介导的种子发芽中的作用.
调查其他植物物种中TeAR介导的DELLA降解的保护.

主要方法:

在不同的光照条件下,对种子发芽表型进行了分析,分析了Arabidopsis thaliana遗传突变 (tear1/2/3/4/5/6,phya-211).
在突变动物中评估了GA1-3 (RGA) 抑制剂的蛋白质积累.
使用 (Physcomitrium patens) 的TEAR同类物 (PpTEAR1,PpTEAR2) 和Arabidopsis DELLAs进行了相互作用和功能测试.

主要成果:

在白色,PhyA和PhyB依赖的光下,Arabidopsis六重撕裂突变体表现出延迟或废除的种子发芽,并积累了RGA.
远红色的光线迅速诱导TEARs,TEAR4作为E3结合酶起作用.
取决于GA的DELLA降解和以TEAR为媒介的DELLA降解都对PhyA依赖的发芽至关重要.
眼同类物与Arabidopsis DELLAs相互作用,它们的过度表达在phya-211突变体中挽救了缺陷的PhyA-依赖发芽.

结论:

眼通过促进DELLA降解来冗余调节种子发芽,作为光信号的关键参与者.
眼介导的DELLA降解是植物王国中保存的古老途径,突出其在发芽过程中的基本作用.
这一途径对于PhyA依赖的发芽是不可或缺的,与GA依赖的降解并肩工作.

关键词:

德拉·德拉拉在这里.E3 结合酶的使用在物理上,PhyA是A.眼蛋白质 (Tear Proteins) 是一种催蛋白质.种子的发芽种子的发芽

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