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优化连接体价值,以最大限度地增加向片向纳米颗粒的肌积累
Emmanuela Adjei-Sowah1,2, Vigneshkumar Rangasami3, Alayna E Loiselle1,2,4,5
1Department of Biomedical Engineering, University of Rochester, Rochester, New York 14623, United States.
ACS applied materials & interfaces
|December 4, 2024
概括
优化纳米粒子连接体密度可以增强肌药物输送. 在纳米颗粒上增加酸抗酸酶 (TRAP) 结合 (TBP) 连接体可以改善肌中的向和保留,从而改善愈合和病理治疗.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 肌肉骨组织工程
背景情况:
- 系统性药物输送到肌肉骨组织 (如肌) 通常是低效的.
- 使用酸抗酸酶 (TRAP) 结合 (TBP) 连接体的向纳米粒子 (NP) 系统此前已开发用于肌愈合.
- 在肌愈合期间,TRAP活动会波动,这表明有时间治疗优化的潜力.
研究的目的:
- 调查纳米颗粒上的变异联结密度是否可以根据TRAP水平优化肌积累.
- 为了确定连接体密度对纳米粒子在肌组织内定位和保留的影响.
- 探索联结体密度调制在肌愈合和病理学中的治疗向的潜力.
主要方法:
- 合成了具有不同TBP连接体密度 (每NP9000-55000) 的多价值纳米粒子.
- 装有三个不同的光体的纳米粒子用于体外和体内追踪.
- 评估了纳米粒子的物理化学性质,TRAP结合亲和力,以及体内肌定位和保留.
主要成果:
- 实验室研究表明,联结体密度与TRAP结合亲和力正相关.
- 纳米粒子的物理化学性质 (大小,电荷,PDI) 没有受到连接体密度或光体的影响.
- 在体内研究表明,连接体密度和纳米粒子在肌中的定位/保留之间存在正相关性.
结论:
- 纳米粒子连接体密度是优化肌准和保留的关键因素.
- 利用带密度提供了一种在肌愈合级联中进行时间治疗的策略.
- 这种方法对治疗肌病理,包括肌病变和与年龄相关的退化有前途.
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